3 years ago

Asymmetric hydrolysis of styrene oxide by PvEH2, a novel Phaseolus vulgaris epoxide hydrolase with extremely high enantioselectivity and regioselectivity

Asymmetric hydrolysis of styrene oxide by PvEH2, a novel Phaseolus vulgaris epoxide hydrolase with extremely high enantioselectivity and regioselectivity
A novel EH from Phaseolus vulgaris, PvEH2, was discovered based on the computer-aided analysis, and its encoding gene was cloned and expressed in E. coli Rossetta (DE3). The substrate spectrum of recombinant (re) PvEH2 was assayed, among which the enantiomeric ratio of rePvEH2 towards racemic styrene oxide (rac-1a) was >200, while its regioselectivity coefficients, α S and β R , towards (S)- and (R)-1a were 99.1 and 69.8%, respectively. The asymmetric hydrolysis of 20mM rac-1a by rePvEH2-expressing whole cells was performed at 25°C, retaining (R)-1a with >99.5% ee s and 49.4% yield and producing (R)-phenyl-1,2-ethanediol (1b) with 96.2% ee p and 49.7% yield in 40min.

Publisher URL: www.sciencedirect.com/science

DOI: S1566736717303667

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