3 years ago

Tuning the pH-Switch of Supramolecular Polymer Carriers for siRNA to Physiologically Relevant pH

Tuning the pH-Switch of Supramolecular Polymer Carriers for siRNA to Physiologically Relevant pH
Matthias Barz, Daniel Spitzer, Hendrik Frisch, Patrick Ahlers, Regina Holm, Pol Besenius
The preparation of histidine enriched dendritic peptide amphiphiles and their self-assembly into multicomponent pH-switchable supramolecular polymers is reported. Alternating histidine and phenylalanine peptide synthons allow the assembly/disassembly to be adjusted in a physiologically relevant range of pH 5.3–6.0. Coassembly of monomers equipped with dendritic tetraethylene glycol chains with monomers bearing peripheral primary amine groups leads to nanorods with a tunable cationic surface charge density. These surface functional supramolecular polycations are able to reversibly bind short interfering RNA (siRNA). The nanorod-like supramolecular polymers, their complexation with siRNA, and the pH-triggered assembly/disassembly of the supramolecular carriers are characterized via circular dichroism spectroscopy, gel electrophoresis, as well as transmission electron microscopy. Multicomponent supramolecular polymers represent a modular and promising strategy for applications as responsive carrier vehicles, codelivery strategies, and gene therapy. Multicomponent self-assembly of dendritic amphiphiles into nanorod-like supramolecular polycations and their reversible complexation with siRNA is reported. Co-assembly of a monomer with tetraethylene glycols with a monomer bearing primary amines leads to supramolecular carriers with a tunable surface charge density. The pH-triggered assembly/disassembly based on a histidine and phenylalanine alternating peptide sequence, is adjusted to a physiologically relevant pH 6.0.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/mabi.201700111

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.