3 years ago

Reversible Bioconjugation: Biodegradable Poly(phosphate)-Protein Conjugates

Reversible Bioconjugation: Biodegradable Poly(phosphate)-Protein Conjugates
Daniela Russo, Greta Becker, Frederik R. Wurm, Tamiris Figueiredo, Tobias Steinbach, Alina Spiegel
Protein-polymer conjugates are widely used to improve the pharmacokinetic properties of therapeutic proteins. Commercially available conjugates employ poly(ethylene glycol) (PEG) as the protective polymer; however, PEG has a number of shortcomings, including non-biodegradability and immunogenicity, that call for the development of alternatives. Here, the synthesis of biodegradable poly(phosphate), that is, poly(ethyl ethylene phosphate) (PEEP), by organo-catalyzed anionic ring-opening polymerization exhibiting dispersity values Ð < 1.3 is reported. Polymers with molecular weights between 2000 and 33 200 g mol−1 are then ω-functionalized with a succinimidyl carbonate group and subsequently conjugated to model proteins. These are the first conjugates based on polyphosphates which degraded upon exposure to phosphodiesterase. As is the case for PEGylated therapeutics, residual in vitro activity of the PPEylated conjugates depends on the extent of protein modification. These results suggest that PEEP exhibits the desired properties of a biopolymer for use in next generation, fully degradable drug delivery systems. A biodegradable alternative to PEGylation: Protein-reactive poly(ethyl ethylene phosphate)s are prepared by anionic ring-opening polymerization and coupled to proteins. These poly(phosphate)-protein conjugates show similar activity to PEGylated analogs and degrade enzymatically.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/mabi.201600377

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