3 years ago

A novel nanoenzyme based on Fe3O4 nanoparticles@thionine-imprinted polydopamine for electrochemical biosensing

A novel nanoenzyme based on Fe3O4 nanoparticles@thionine-imprinted polydopamine for electrochemical biosensing
Here, a new nanoenzyme of Fe3O4 nanoparticles (NPs) magnetic molecularly imprinted polymers (MMIPs) was prepared by polymerizing dopamine on the Fe3O4NPs surface in the presence of templated thionine (Thi) for the first time. The results showed that uniform spherical and core-shell structured Fe3O4NPs MMIPs which were about 600nm in diameter were successfully formed and the imprinting sites improved the selectivity of Fe3O4NPs MMIPs greatly. The as-prepared Fe3O4NPs MMIPs could catalyze the reduction of Thi selectively, which could be enhanced by H2O2 owing to the peroxidase-like activity of Fe3O4NPs. Accordingly, a highly selective and sensitive H2O2 electrochemical biosensor was proposed based on the Fe3O4NPs MMIPs-modified glassy carbon electrode. The electrochemical biosensor based on the Fe3O4NPs MMIPs nanoenzyme exhibited low detection limit of 1.58nM and high selectivity. Since acetylthiocholine chloride (AChl) could be hydrolyzed into choline with the help of acetylcholinesterase (AChE) and simultaneously the choline oxidase (ChOx) could reduce choline into betaine accompanied by the production of H2O2, the proposed electrochemical H2O2 biosensor could be further used to detect AChl, AChE and ChOx. The results also exhibited wide linear range (2.85–160μM for AChl, 0.53–20000ngmL−1 for AChE and 22.76–400ngmL−1 for ChOx), low detection limit (0.86μM for AChl, 0.16ngmL−1 for AChE and 6.83ngmL−1 for ChOx) and high selectivity. Therefore, the Fe3O4NPs MMIPs should be a promising nanoenzyme for electrochemical biosensors.

Publisher URL: www.sciencedirect.com/science

DOI: S0925400517311516

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.