Analytica Chimica Acta
Analytica Chimica Acta
5 years ago

Study of antioxidant effects on malignant glioma cells by constructing a tumor-microvascular structure on microchip

Study of antioxidant effects on malignant glioma cells by constructing a tumor-microvascular structure on microchip
In this work, a three-dimensional tumor-microvascular structure was simulated on a microfluidic chip for study of antioxidants effects on malignant glioma cells in vitro. The 3D hydrogel containing lumen was constructed to co-culture endothelial cells and glioma cells to mimic tumor microvascular environment. Macroporous gelatin transglutaminase (TG) hydrogel was prepared with biological and mechanical properties suitable for cells culture and nutrient refresh. To reform a vessel structure, U87 cells were dispersed in the TG-gelatin hydrogel and HUVEC cells were seeded in the lumen of hydrogel. Three typical antioxidants (α-lipoic acid, catechins and ascorbic acid) have been selected to research the antioxidant effects of glioma cells in the simulative tumor microenvironment. The results showed that the HUVEC cells formed vessel presented the transportation and penetrable functions for antioxidants from lumen to glioma cells. The antioxidants displayed higher selectivity to U87 cells than HUVEC cells and α-lipoic acid has a strong antioxidant capacity.

Publisher URL: www.sciencedirect.com/science

DOI: S0003267017305858

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.