3 years ago

An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues

An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues
Maya Kasowski, Beijing Wu, Adam J Rubin, Paul A Khavari, Emily G Hamilton, Seung Woo Cho, Howard Y Chang, Nicholas A Sinnott-Armstrong, Ansuman T Satpathy, Maxwell R Mumbach, Ava C Carter, Lisa A Orloff, Peyton G Greenside, Alexandro E Trevino, Sam Vesuna, Viviana I Risca, Thomas J Montine, Kathleen S Montine, Anshul Kundaje, M Ryan Corces, William J Greenleaf, Arwa Kathiria
We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol generates chromatin accessibility profiles from archival frozen tissue samples and 50-μm sections, revealing the activities of disease-associated DNA elements in distinct human brain structures. The Omni-ATAC protocol enables the interrogation of personal regulomes in tissue context and translational studies.

Publisher URL: http://dx.doi.org/10.1038/nmeth.4396

DOI: 10.1038/nmeth.4396

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