3 years ago

Sampling strategies to capture single-cell heterogeneity

Sampling strategies to capture single-cell heterogeneity
John D Gordan, Satwik Rajaram, Steven J Altschuler, Agnieszka K Witkiewicz, Kathy M Bonness, James S Malter, Chloe E Atreya, Jayant Avva, Robert S Warren, Lani F Wu, Louise E Heinrich
Advances in single-cell technologies have highlighted the prevalence and biological significance of cellular heterogeneity. A critical question researchers face is how to design experiments that faithfully capture the true range of heterogeneity from samples of cellular populations. Here we develop a data-driven approach, illustrated in the context of image data, that estimates the sampling depth required for prospective investigations of single-cell heterogeneity from an existing collection of samples.

Publisher URL: http://dx.doi.org/10.1038/nmeth.4427

DOI: 10.1038/nmeth.4427

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