3 years ago

Lactobacillus rhamnosus L34 attenuates gut translocation induced bacterial sepsis in murine models of leaky gut.

Asada Leelahavanichkul, Wimonrat Panpetch, Wiwat Chancharoenthana, Kanthika Bootdee, Somying Tumwasorn, Malcolm Finkelman, Sumanee Nilgate
Gastrointestinal (GI) bacterial translocation in sepsis is well-known but the role of the probiotics, Lactobacillus spp., is still controversial. We evaluated the therapeutic effects of Lactobacillus rhamnosus L34 in a new sepsis model of oral administration of pathogenic bacteria, with GI leakage induced by either an antibiotic cocktail (ATB) and/or dextran sulfate sodium (DSS).GI-leakage with ATB, DSS and DSS+ATB was demonstrated by FITC-dextran translocation to the circulation. Administration of pathogenic bacteria, either Klebsiella pneumoniae or Salmonella enterica serovar Typhimurium, enhanced the translocation. Bacteremia was demonstrated within 24h in 50-88% of mice with GI leakage plus pathogenic bacteria administration but not with GI-leakage induction alone or bacterial gavage alone. Salmonella bacteremia was found in only 16-29% and 0% of mice with Salmonella and Klebsiella administration, respectively. Klebsiella bacteremia was demonstrated in 25-33% and 10-16% of mice with Klebsiella and Salmonella administration, respectively.Lactobacillus rhamnosus L34 attenuated GI leakage in these models, as shown by the reduction of FITC-dextran gut-translocation, serum IL-6, bacteremia and sepsis mortality. The reduction of fecal Salmonella was demonstrated with Lactobacillus treatment. In addition, an anti-inflammatory effect of the conditioned media from Lactobacillus rhamnosus L34 was also demonstrated by the attenuation of cytokine production in colonic-epithelial cells in vitroIn conclusion, Lactobacillus rhamnosus L34 attenuated the severity of symptoms in a murine sepsis model induced by GI leakage and pathogenic bacterial administration.

Publisher URL: http://doi.org/10.1128/IAI.00700-17

DOI: 10.1128/IAI.00700-17

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.