This July, we are introducing our Researcher Live mini-series on ‘Applications of CRISPR’! Join our second episode on 19th July at 10am BST / 9am GMT for a discussion with Dr Jordi Cayuso, University of Wolverhampton. Sign up here to receive email reminders for this series.
What are we going to talk about in this episode?
Zebrafish is an excellent model for the study of embryonic development, regeneration and disease. However, due to the inefficiency of homologous recombination, reverse genetics approaches in this organism have been technically challenging. In recent years, CRISPR/Cas9 genome-editing has opened many new research avenues in fundamental biology. Critically, in zebrafish research it provided us a straightforward way to mutate genes of interest, a key step in gene function analysis and disease modelling. In this talk, I will introduce CRISPR/Cas9 technology and show the findings that led us to an optimised experimental pipeline for genome editing zebrafish. Using this technique we are now able to efficiently introduce mutations in the genome and assess gene function in less than one week.
Please follow Researcher Live’s profile ‘CRISPR Applications’ for updates on the topic and events!
Series programme:
- 11th July, 4pm BST / 3pm GMT - ‘Introduction to CRISPR, a family of proteins that is revolutionising genome editing’ with Dr Elisa Aguilar-Martinez, University of Manchester
- 19th July, 10am BST/ 9am BST - ‘Using CRISPR/Cas9 to Understand Gene Function in Zebrafish’ with Dr Jordi Cayuso, University of Wolverhampton.
What are we going to talk about in this episode?
This talk will introduce the audience to CRISPR-Cas proteins, focusing on their application in the fight against diseases and why they are now indispensable as a researching tool. It will specifically explore how we use these proteins, covering experimental models of diseases, control of gene expression and diagnosis of viruses and other pathogens. The advantages and disadvantages of the different Cas proteins will also be discussed as well as the things to consider before starting working with CRISPR as a practical 'how to' guide.
Please follow Researcher Live’s profile ‘CRISPR Applications’ for updates on the topic and events!
Series programme:
- 11th July, 4pm BST / 3pm GMT - ‘Introduction to CRISPR, a family of proteins that is revolutionising genome editing’ with Dr Elisa Aguilar-Martinez, University of Manchester
- 19th July, 10am BST/ 9am BST - ‘Using CRISPR/Cas9 to Understand Gene Function in Zebrafish’ with Dr Jordi Cayuso, University of Wolverhampton
If you'd like to present at your own Researcher Live event, please email kristine.lennie@researcher-app.com
Dr Jordi Cayuso, University of Wolverhampton
Jordi is a cell and developmental biologist interested in understanding how cell-to-cell communication controls cell behaviour during embryonic development and tissue regeneration. During his Phd Jordi uncovered how the secreted protein Sonic Hedgehog coordinates the patterning, growth and differentiation of neural precursors in the vertebrate neural tube.
After joining David Wilkinson laboratory at The Francis Crick Institute (London), Jordi investigated the role of Eph/Ephrin signalling in the formation of tissue boundaries, a critical process in embryonic development and disease. For this, he established knock- out and knock-in CRISPR/Cas9 genome-editing strategies and generated null and signalling-deficient Eph/Ephrin zebrafish lines. Using these tools, he uncovered that the Eph receptor coordinates the formation of sharp borders with the acquisition of cell identities by regulating the mechanical properties of cells and activating Yap/Taz mechanotransduction.
In December 2020 Jordi joined the Faculty of Science and Engineering at University of Wolverhampton as a Senior Lecturer in Reproduction and Developmental Biology
DOI: wpQaQzT7IBgkvm0U8x8k_prepost_1
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