In our next Researcher Live series this summer, we will be focusing on ‘Applications of Mass Spectrometry’ – bringing you four fantastic speakers over the course of July and early August.
Join our first episode on 20th July at 10am BST / 9am GMT with Dr Mariya Misheva, University of Oxford. Sign up here to receive email reminders for this series.
What are we going to talk about in this episode?
The role of microbially-derived polar and ionic metabolites, e.g., short-chain fatty acids (SCFA), is of considerable interest in studying microbiome-host relationships in the context of health and disease. Detection and quantification of SCFAs in biological matrices, such as blood, urine and faecal samples, remains challenging in part due to low selectivity, high compound polarity, low abundance and volatility.
IC-MS enables separation, detection and quantification of biologically relevant SCFA and their isomers, including a wide range of additional organic acids, relevant to biological sample including blood plasma and faecal extracts. Therefore, IC-MS methods can be used for analysis of microbiome-related samples in both targeted and untargeted metabolomics experiments. Importantly, IC-MS offers minimal sample preparation without the need for derivatisation is needed. Thus, IC-MS can successfully detect and quantify microbiome-related volatile and non-volatile metabolites.
Series programme:
- 20th July, 10am BST / 9am GMT - ‘A new method for the analysis of short-chain fatty acids (SCFA) and other polar metabolites in microbiome-related samples by ion-exchange chromatography-mass spectrometry (IC-MS)’ with Dr Mariya Misheva, University of Oxford
- 21st July, 4pm BST/ 3pm GMT - ‘Building a Versatile Intact Mass Toolkit to Improve Integrative Protein Structure Characterization for Drug Development and Basic Research’ with Dr Aaron Bailey, The University of Texas Medical Branch
- 28th July, 10am BST/9am GMT – ‘Mass Spectrometry-based bottom-up proteomics and its applications in cancer research: an overview’ with Dr Roopesh Krishnankutty, Senior Mass Spectrometry Researcher at the University of Edinburgh
- 2nd August, 10am BST/9am GMT – ‘Determining mass, why it is important and why it matters?’ with Dr Gillian Taylor - 2nd August 10am BST, University of Newcastle-upon-Tyne
Please follow Researcher Live’s profile ‘Applications of Mass Spectrometry’ for updates on the topic and to keep up with future event series!
If you'd like to present at your own Researcher Live event, please email kristine.lennie@researcher-app.com
Dr Mariya Misheva, University of Oxford
Dr Mariya Misheva is a PDRA in Microbiome-related Metabolomics in the group of Professor James McCullagh, Director of Mass Spectrometry Facility, University of Oxford. Her position is funded by the Oxford Centre for Microbiome Studies (OCMS) team, and her research focuses on developing new metabolomics methods and analysis pipelines to accelerate researching the role of the microbiome and its metabolome in health and disease.
She received her BSc and MSc degrees from the University of Sofia, Bulgaria. Afterwards, she was awarded a PhD scholarhip by the University of Melbourne, Australia where she successfully completed her studies under the supervision of A/ Prof. Marie Bogoyevitch. She performed her postdoctoral studies first at Cardiff University as part of the Lipidomics group led by Prof. Valerie O'Donnell, and thenshe joined the OCMS team at the University of Oxford.
Mariya’s interest in mass spectrometry started during her PhD project where she used proteomics to investigate c-Jun N-terminal kinase (JNK1) post-translational modifications and protein complexes under basal and stress conditions. In the group of Valerie O’Donnell my work has focused on elucidating the role of lipids in innate immunity, more specifically eicosanoid production by microphages in inflammation. Lastly, my current position involves microbiome-related metabolomics projects with an emphasis on the gut microbiome metabolome and its role in healthy lifestyle and disease progression.
Her career objectives are to increase her expertise in mass spectrometry and to use her experience to develop new tools, methods and protocols for solving complex biological problems.
DOI: WTkDVwP5ykglqxhlyP5X_prepost_1
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