3 years ago

Mechanisms of phosphorus acquisition and lipid class remodelling under P limitation in a marine microalga.

Alice Mühlroth, Atle M Bones, Aimen El Assimi, Martin F Hohmann-Marriott, Juliette Jouhet, Per Winge, Olav Vadstein, Eric Marechal
Molecular mechanisms of phosphorus (P) limitation are of great interest for understanding algal production in aquatic ecosystems. Previous studies point to P limitation induced changes in lipid composition. As in microalgae the molecular mechanisms of this specific P-stress adaptation remain unresolved, we reveal a detailed phospholipid-recycling scheme in Nannochloropsis oceanica and described important P acquisition genes based on highly corresponding transcriptome and lipidome data. Initial responses to P limitation showed increased expression of genes involved in P uptake and an expansion of P substrate spectrum based on purple acid phosphatases. Increase in P trafficking displayed rearrangement between compartments by supplying P to the chloroplast and carbon to the cytosol for lipid synthesis. We propose a novel phospholipid-recycling scheme for algae that leads to rapid reduction of phospholipids and synthesis of phosphorus-free lipid classes. P-mobilization through membrane lipid degradation is mediated mainly by two glycerophosphoryldiester phosphodiesterases and three patatin-like phospholipases A on transcriptome level. To compensate low phospholipids, in the exponential growth, Nannochloropsis synthesized sulfoquinovosyl diacylglycerol and diacylglyceroltrimethylhomoserine. In this study it was shown that N. oceanica strain has a unique repertoire of genes that facilitate P acquisition and degradation of phospholipids compared to other stramenopiles. The novel phospholipid-recycling scheme opens new avenues for metabolic engineering of lipid composition in algae.

Publisher URL: http://doi.org/10.1104/pp.17.00621

DOI: 10.1104/pp.17.00621

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