Talanta
Talanta
4 years ago

Novel metal nanoparticle-enhanced fluorescence for determination of trace amounts of fluoroquinolone in aqueous solutions

Novel metal nanoparticle-enhanced fluorescence for determination of trace amounts of fluoroquinolone in aqueous solutions
Metal-enhanced fluorescence of fluoroquinolones (FQs) was first observed in aqueous solutions. In addition, a new type of silver nanoparticles (AgNP) was synthesized with simple and easy synthetic processes and environmentally friendly compounds. The effects of different concentrations of AgNPs on the fluorescence behaviours of ciprofloxacin (CIP), enrofloxacin (ENR) and lomefloxacin (LMF) in aqueous solutions were investigated, respectively. The experimental results demonstrated that the fluorescence peak shapes and the locations of the features in the excitation and emission spectra for each FQ that was mixed with AgNPs were almost the same as those of the standard FQs. An enhancement or quenching of the fluorescence can also be observed, depending on the exact conditions. Compared with the identical control samples that lack AgNPs, the fluorescence of each FQ in aqueous solutions was greatly enhanced by AgNPs with concentrations at a volume ratio of 5%. Moreover, at the optimum AgNP concentration, novel sensitive fluorometric methods for the separate determination of trace amounts of CIP, ENR and LMF in aqueous solutions were established. Under optimal experimental conditions, the linear dynamic ranges for the determination of CIP, ENR and LMF concentrations varied from 0.025 to 1.0mgL−1, 5.0 to 160ngL−1 and 0.01 to 0.8mgL−1, and the limits of detection were 90, 5 and 6ngL−1, respectively; the relative standard deviation was less than 1.2% (n = 9). The experimental recovery results for the determination of CIP, ENR and LMF in aqueous solutions ranged from 99% to 102%, 90% to 103% and 92% to 107%, respectively. Compared with the established method in which no AgNPs were added, the quantitation limits of the silver nanoparticle-enhanced fluorometric methods were approximately 2-fold lower for CIP, 2.6-fold lower for ENR and 4-fold lower for LMF. Significantly, the novel silver nanoparticle-enhanced fluorometric methods were successfully applied to directly determine CIP, ENR and LMF concentrations in pharmaceutical preparations, demonstrating the methods’ advantages of simplicity, sensitivity and low cost.

Publisher URL: www.sciencedirect.com/science

DOI: S0039914017305805

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.