3 years ago

Combined platelet-rich plasma and lipofilling treatment provides great improvement in facial skin-induced lesion regeneration for scleroderma patients

Valentina Caputo, Giuseppe Pistone, Maria Luisa Colorito, Tiziana Apuzzo, Francesco Dieli, Francesco Virzì, Roberto Pirrello, Laura Rosa Mangiapane, Emanuela Scavo, Simone Di Franco, Dario Catalano, Antonina Benfante, Annalisa Nicotra, Alessandro Giammona, Giorgio Stassi, Paola Bianca

Abstract

Background

The use of stem cells, including mesenchymal stem cells (MSCs), for regenerative medicine is gaining interest for the clinical benefits so far obtained in patients. This study investigates the use of adipose autologous tissue in combination with platelet-rich plasma (PRP) to improve the clinical outcome of patients affected by systemic sclerosis (SSc).

Methods

Adipose-derived mesenchymal stem cells (AD-MSCs) and PRPs were purified from healthy donors and SSc patients. The multilineage differentiation potential of AD-MSCs and their genotypic–phenotypic features were investigated. A cytokine production profile was evaluated on AD-MSCs and PRPs from both healthy subjects and SSc patients. The adipose tissue-derived cell fraction, the so-called stromal vascular fraction (SVF), was coinjected with PRP in the perioral area of SSc patients.

Results

Histopathological and phenotypical analysis of adipose tissue from SSc patients revealed a disorganization of its distinct architecture coupled with an altered cell composition. Although AD-MSCs derived from SSc patients showed high multipotency, they failed to sustain a terminally differentiated progeny. Furthermore, SVFs derived from SSc patients differed from healthy donors in their MSC-like traits coupled with an aberrant cytokine production profile. Finally, the administration of PRP in combination with autologous SVF improved buccal’s rhyme, skin elasticity and vascularization for all of the SSc patients enrolled in this study.

Conclusions

This innovative regenerative therapy could be exploited for the treatment of chronic connective tissue diseases, including SSc.

Publisher URL: https://link.springer.com/article/10.1186/s13287-017-0690-3

DOI: 10.1186/s13287-017-0690-3

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