3 years ago

Peptide Logic Circuits Based on Chemoenzymatic Ligation for Programmable Cell Apoptosis

Peptide Logic Circuits Based on Chemoenzymatic Ligation for Programmable Cell Apoptosis
Shanfang Hu, Zhou Nie, Ke Zhang, Lin Fan, Yong Li, Yan Huang, Shouzhou Yao, Sujuan Sun
A novel and versatile peptide-based bio-logic system capable of regulating cell function is developed using sortase A (SrtA), a peptide ligation enzyme, as a generic processor. By modular peptide design, we demonstrate that mammalian cells apoptosis can be programmed by peptide-based logic operations, including binary and combination gates (AND, INHIBIT, OR, and AND-INHIBIT), and a complex sequential logic circuit (multi-input keypad lock). Moreover, a proof-of-concept peptide regulatory circuit was developed to analyze the expression profile of cell-secreted protein biomarkers and trigger cancer-cell-specific apoptosis. It's only bio-logical: A versatile peptide-based bio-logic system has been developed by using sortase A (SrtA), an emerging peptide ligation enzyme, as a generic processor. By modular design of peptide inputs, cancer cells apoptosis can be programmed by peptide logic circuits.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201708327

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