5 years ago

Dynamic Modulation of Enzyme Activity by Near-Infrared Light

Dynamic Modulation of Enzyme Activity by Near-Infrared Light
Qiang Zhang, Xinyu Wang, Jianhua Xu, Changping Wang, Ruijuan Qi, Yuankai Tang, Yiyun Cheng, Sanjun Zhang, Hong Chang
Engineering near-infrared (NIR) light-sensitive enzymes remains a huge challenge. A photothermal effect-associated method is developed for tailoring the enzymatic activity of enzymes by exposure to NIR light. An ultrasmall platinum nanoparticle was anchored in an enzyme to generate local heating upon NIR irradiation, which enhanced the enzyme activity without increasing bulk temperature. Following NIR irradiation, the enzyme activity was tailored rapidly and reversibly, and was modulated by varying laser power density and irradiation time. Four enzymes were engineered, including glucoamylase, glucose oxidase, catalase, and proteinase K with NIR-light sensitivity, and demonstrated their utility in practical applications such as photolithography and NIR light-responsive antibacterial or anticancer actions. Our investigation suggests that this approach could be broadly used to engineer enzymes with NIR-light sensitivity for many biological applications. Four different enzymes (brown) anchored by platinum nanoparticles (gray sphere) showed enhanced enzymatic activity by NIR irradiation (right) over that without irradiation (left). Glucoamylase, glucose oxidase, catalase, and proteinase K enzymes were studied. Blue=substrate, red+=product.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201700968

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