3 years ago

A CRISPR screen identifies a pathway required for paraquat-induced cell death

A CRISPR screen identifies a pathway required for paraquat-induced cell death
Navdeep S Chandel, Kıvanç Birsoy, Tim Wang, Peng Gao, Inmaculada Martínez-Reyes, Hyewon Kong, David M Sabatini, Colleen R Reczek
Paraquat, a herbicide linked to Parkinson's disease, generates reactive oxygen species (ROS), which causes cell death. Because the source of paraquat-induced ROS production remains unknown, we conducted a CRISPR-based positive-selection screen to identify metabolic genes essential for paraquat-induced cell death. Our screen uncovered three genes, POR (cytochrome P450 oxidoreductase), ATP7A (copper transporter), and SLC45A4 (sucrose transporter), required for paraquat–induced cell death. Furthermore, our results revealed POR as the source of paraquat-induced ROS production. Thus, our study highlights the use of functional genomic screens for uncovering redox biology.

Publisher URL: https://www.nature.com/articles/nchembio.2499

DOI: 10.1038/nchembio.2499

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