3 years ago

Rat embryonic stem cells produce fertile offspring through tetraploid complementation [Developmental Biology]

Rat embryonic stem cells produce fertile offspring through tetraploid complementation [Developmental Biology]
Jia-Qiang Wang, Cong Wan, Yu-Fei Li, Xin-Xin Zhang, Jun-Jie Mao, Bao-Yang Hu, Xin Li, Le-Yun Wang, Bin Qu, Wei Li, Xiao-Yang Zhao, Tong-Tong Cui, Gui-Hai Feng, Qi Zhou, Mei Wang, Lei Liu, Tian-Da Li, Hai-Feng Wan, Xue-Peng Wang

Pluripotency of embryonic stem cells (ESCs) can be functionally assessed according to the developmental potency. Tetraploid complementation, through which an entire organism is produced from the pluripotent donor cells, is taken as the most stringent test for pluripotency. It remains unclear whether ESCs of other species besides mice can pass this test. Here we show that the rat ESCs derived under 2i (two small molecule inhibitors) conditions at very early passages are able to produce fertile offspring by tetraploid complementation. However, they lose this capacity rapidly during culture due to a nearly complete loss of genomic imprinting. Our findings support that the naïve ground state pluripotency can be captured in rat ESCs but also point to the species-specific differences in its regulation and maintenance, which have implications for the derivation and application of naïve pluripotent stem cells in other species including human.

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