Discovery of Novel Potent Reversible and Irreversible Myeloperoxidase Inhibitors Using Virtual Screening Procedure

5 years ago

Discovery of Novel Potent Reversible and Irreversible Myeloperoxidase Inhibitors Using Virtual Screening Procedure

Jörg Flemmig, Annelise De Carvalho, Iyas Aldib, Paul G. Furtmüller, François Dufrasne, Pierre Van Antwerpen, Martine Prévost, Christian Obinger, Michel Gelbcke, Jalal Soubhye, Ibaa Chikh Alard, Karim Zouaoui Boudjeltia, Véronique Mathieu, Jean Nève, Alexandre Rousseau, Franck Meyer, Sara Tadrent

The heme enzyme myeloperoxidase (MPO) participates in innate immune defense mechanism through formation of microbicidal reactive oxidants. However, evidence has emerged that MPO-derived oxidants contribute to propagation of inflammatory diseases. Because of the deleterious effects of circulating MPO, there is a great interest in the development of new efficient and specific inhibitors. Here, we have performed a novel virtual screening procedure, depending on ligand-based pharmacophore modeling followed by structure-based virtual screening. Starting from a set of 727842 compounds, 28 molecules were selected by this virtual method and tested on MPO in vitro. Twelve out of 28 compounds were found to have an IC₅₀ less than 5 μM. The best inhibitors were 2-(7-methoxy-4-methylquinazolin-2-yl)guanidine (28) and (R)-2-(1-((2,3-dihydro-1H-imidazol-2-yl)methyl)pyrrolidin-3-yl)-5-fluoro-1H-benzo[d]imidazole (42) with IC₅₀ values of 44 and 50 nM, respectively. Studies on the mechanism of inhibition suggest that 28 is the first potent mechanism-based inhibitor and inhibits irreversibly MPO at nanomolar concentration.

Publisher URL: http://dx.doi.org/10.1021/acs.jmedchem.7b00285

DOI: 10.1021/acs.jmedchem.7b00285