Brice Felden, Fabrice Journé, Petra El Hajj, Ghanem Ghanem, Jean-Christophe Marine, Laura Bachelot, Rania Ben Jouira, Sébastien Corre, David Gilot, Florian Rambow, Nicolas Mouchet, Bernard Mari, Marie-Laure Pinel-Marie, Aljosja Rogiers, Marie-Dominique Galibert, Emmanuelle Donnou-Fournet, Ariane Mogha, Mélodie Migault, Sophie Tartare-Deckert, Arthur Gautron, Tristan Montier
Competition among RNAs to bind miRNA is proposed to influence biological systems. However, the role of this competition in disease onset is unclear. Here, we report that TYRP1 mRNA, in addition to encoding tyrosinase-related protein 1 (TYRP1), indirectly promotes cell proliferation by sequestering miR-16 on non-canonical miRNA response elements. Consequently, the sequestered miR-16 is no longer able to repress its mRNA targets, such as RAB17, which is involved in melanoma cell proliferation and tumour growth. Restoration of miR-16 tumour-suppressor function can be achieved in vitro by silencing TYRP1 or increasing miR-16 expression. Importantly, TYRP1-dependent miR-16 sequestration can also be overcome in vivo by using small oligonucleotides that mask miR-16-binding sites on TYRP1 mRNA. Together, our findings assign a pathogenic non-coding function to TYRP1 mRNA and highlight miRNA displacement as a promising targeted therapeutic approach for melanoma.