3 years ago

Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV

Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV
Gabriel C Lander, Mengyu Wu, Mark A Herzik Jr
Nearly all single-particle cryo-EM structures resolved to better than 4-Å resolution have been determined using 300-keV transmission electron microscopes (TEMs). We demonstrate that it is possible to obtain reconstructions of macromolecular complexes of different sizes to better than 3-Å resolution using a 200-keV TEM. These structures are of sufficient quality to unambiguously assign amino acid rotameric conformations and identify ordered water molecules.

Publisher URL: https://www.nature.com/articles/nmeth.4461

DOI: 10.1038/nmeth.4461

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