3 years ago

Excess Electron Transfer through DNA Duplexes Comprising a Metal-Mediated Base Pair

Excess Electron Transfer through DNA Duplexes Comprising a Metal-Mediated Base Pair
Jens Müller, Nicole Megger, Uwe Karst, Kevin Eckey, Susanne Hensel, Philipp Scharf
Excess electron transfer through one set of DNA duplexes comprising either one or two metal-mediated thymine–HgII–thymine base pairs was studied. Towards this end, the metal-mediated base pair(s) were introduced between an artificial nucleoside bearing a N,N,N′,N′-tetramethyl-1,5-diaminonaphthalene derivative (dN, acting as a photoinducible electron donor) and 5-bromo-2′-deoxyuridine (dB, acting as an electron acceptor). Upon one-electron reduction, dB loses a bromide ion. The amount of unreacted dB remaining after irradiation—determined by LC/ICP-MS—was used to evaluate the charge-transfer efficiency across the metal-mediated base pair(s). Reference measurements with canonical adenine:thymine base pairs prove the applicability of this approach for the detection of charge transfer in DNA. The data indicate that, for the set of DNA duplexes under investigation, excess electron transfer across a thymine–HgII–thymine base pair proceeds with low efficiency, comparable to the transfer across a thymine:thymine mispair. Two contiguous thymine–HgII–thymine base pairs effectively shut down excess electron transfer. A new method for an evaluation of the efficiency of excess electron transfer through DNA duplexes is introduced. Its application to the study of charge transfer across a thymine–HgII–thymine base pair indicates a significant decline compared with a canonical base pair (see scheme).

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/chem.201702241

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.