3 years ago

Phosphorylation of Aspergillus fumigatus PkaR Impacts Growth and Cell Wall Integrity Through Novel Mechanisms

William J. Steinbach, M. Arthur Moseley, Greg Waitt, E. Keats Shwab, Nathan I. Nicely, Praveen R. Juvvadi, Erik J. Soderblom
PKA signaling is essential for growth and virulence of the fungal pathogen Aspergillus fumigatus. Little is known concerning the regulation of this pathway in filamentous fungi. Employing LC-MS/MS, we identified novel phosphorylation sites on the regulatory subunit PkaR, distinct from those previously identified in mammals and yeasts, and demonstrated the importance of two phosphorylation clusters for hyphal growth and cell wall stress response. We also identified key differences in the regulation of PKA subcellular localization in A. fumigatus compared to other species. This is the first analysis of the phosphoregulation of a PKA regulatory subunit in a filamentous fungus and uncovers critical mechanistic differences between PKA regulation in filamentous fungi compared to mammals and yeast species, suggesting divergent targeting opportunities. This article is protected by copyright. All rights reserved.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/1873-3468.12886

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