3 years ago

Alkali-Mediated Miscibility of Gelatin/Polycaprolactone for Electrospinning Homogeneous Composite Nanofibers for Tissue Scaffolding

Alkali-Mediated Miscibility of Gelatin/Polycaprolactone for Electrospinning Homogeneous Composite Nanofibers for Tissue Scaffolding
Huihua Yuan, Yanzhong Zhang, Qihui Zhou, Bei Feng, Patrick Rijn, Zhepao Yu, Ya Zhou, Huilan Zhang
Electrospun natural-synthetic composite nanofibers, which possess favorable biological and mechanical properties, have gained widespread attention in tissue engineering. However, the development of biomimetic nanofibers of hybrids remains a huge challenge due to phase separation of the polymer blends. Here, aqueous sodium hydroxide (NaOH) solution is proposed to modulate the miscibility of a representative natural-synthetic hybrid of gelatin (GT) and polycaprolactone (PCL) for electrospinning homogeneous composite nanofibers. Alkali-doped GT/PCL solutions and nanofibers examined at macroscopic, microscopic, and internal molecular levels demonstrate appropriate miscibility of GT and PCL after introducing the alkali dopant. Particularly, homogeneous GT/PCL nanofibers with smooth surface and uniform diameter are obtained when aqueous NaOH solution with a concentration of 10 m is used. The fibers become more hydrophilic and possess improved mechanical properties both in dry and wet conditions. Moreover, biocompatibility experiments show that stem cells adhere to and proliferate better on the alkali-modified nanofibers than the untreated one. This study provides a facile and effective approach to solve the phase separation issue of the synthetic-natural hybrid GT/PCL and establishes a correlation of compositionally and morphologically homogeneous composite nanofibers with respect to cell responses. Aqueous sodium hydroxide solution is used to modulate the miscibility of gelatin and polycaprolactone for electrospinning morphologically uniform and compositionally homogeneous composite nano­fibers, which exhibit more hydrophilic, improved mechanical properties both in dry and wet conditions, and interact favorably with the murine induced pluripotent stem cell-derived mesenchymal stem cells as compared to the untreated one.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/mabi.201700268

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