3 years ago

Three-Dimensional Printing of Nano Hydroxyapatite/Poly(ester urea) Composite Scaffolds with Enhanced Bioactivity

Three-Dimensional Printing of Nano Hydroxyapatite/Poly(ester urea) Composite Scaffolds with Enhanced Bioactivity
Matthew L. Becker, Gabrielle V. Seifert, Yanyi Xu, Jiayi Yu, Shan Li
Polymer–bioceramic composites incorporate the desirable properties of each material while mitigating the limiting characteristics of each component. 1,6-Hexanediol l-phenylalanine-based poly(ester urea) (PEU) blended with hydroxyapatite (HA) nanocrystals were three-dimensional (3D) printed into porous scaffolds (75% porosity) via fused deposition modeling and seeded with MC3T3-E1 preosteoblast cells in vitro to examine their bioactivity. The resulting 3D printed scaffolds exhibited a compressive modulus of ∼50 MPa after a 1-week incubation in PBS at 37 °C, cell viability >95%, and a composition-dependent enhancement of radio-contrast. The influence of HA on MC3T3-E1 proliferation and differentiation was measured using quantitative real-time polymerase chain reaction, immunohistochemistry and biochemical assays. After 4 weeks, alkaline phosphatase activity increased significantly for the 30% HA composite with values reaching 2.5-fold greater than the control. Bone sialoprotein showed approximately 880-fold higher expression and 15-fold higher expression of osteocalcin on the 30% HA composite compared to those of the control. Calcium quantification results demonstrated a 185-fold increase of calcium concentration in mineralized extracellular matrix deposition after 4 weeks of cell culture in samples with higher HA content. 3D printed HA-containing PEU composites promote bone regeneration and have the potential to be used in orthopedic applications.

Publisher URL: http://dx.doi.org/10.1021/acs.biomac.7b01222

DOI: 10.1021/acs.biomac.7b01222

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