3 years ago

Visualizing the Adenylation Activities and Protein–Protein Interactions of Aryl Acid Adenylating Enzymes

Visualizing the Adenylation Activities and Protein–Protein Interactions of Aryl Acid Adenylating Enzymes
Shota Kasai, Fumihiro Ishikawa, Genzoh Tanabe, Hideaki Kakeya
The cover picture shows protein–protein interactions between aryl acid carrier proteins (green) and aryl acid adenylation (A) domains (blue) that were visualized and assessed by using a new gel-based strategy coupled with activity-based probes. The substrate specificities of these A-domains are also shown. This strategy should be immediately useful for assessing the nature of transient and specific CP–partner interactions and manipulating protein–protein interactions between non-native pairs. More information can be found in the communication by F. Ishikawa et al.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/cbic.201700566

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