3 years ago

A Reversible Fluorescent Probe for Real-Time Quantitative Monitoring of Cellular Glutathione

A Reversible Fluorescent Probe for Real-Time Quantitative Monitoring of Cellular Glutathione
Xue Wu, Nahyun Kwon, Ying Hu, Juyoung Yoon, Zhixue Liu, Xin Zhou, Yu Miao
The ability to monitor and quantify glutathione (GSH) in live cells is essential in order to gain a detailed understanding of GSH-related pathological events. However, owing to their irreversible response mechanisms, most existing fluorescent GSH probes are not suitable for this purpose. We have developed a ratiometric fluorescent probe (QG-1) for quantitatively monitoring cellular GSH. The probe responds specifically and reversibility to GSH with an ideal dissociation constant (Kd) of 2.59 mm and a fast response time (t1/2=5.82 s). We also demonstrate that QG-1 detection of GSH is feasible in a model protein system. QG-1 was found to have extremely low cytotoxicity and was applied to determine the GSH concentration in live HeLa cells (5.40±0.87 mm). Given the green light: A ratiometric fluorescent probe (QG-1) for monitoring and quantifying variations in cellular glutathione (GSH) was developed. The probe shows a fluorescence shift from red to green upon binding GSH and exhibits specificity and reversibility, with an appropriate dissociation constant for sensing species with high cellular abundance (Kd=2.59 mm) and a fast response time (t1/2=5.82 s).

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201702114

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