Industrial & Engineering Chemistry Research
Industrial & Engineering Chemistry Research
5 years ago

Equilibrium Theory Based Design Space for the Multicolumn Countercurrent Solvent Gradient Purification Process

Equilibrium Theory Based Design Space for the Multicolumn Countercurrent Solvent Gradient Purification Process
Fabian Steinebach, Giuseppe Storti, Martin Krättli, Massimo Morbidelli
A procedure for designing the operation parameter space for the twin-column multicolumn countercurrent solvent gradient purification (MCSGP) process for the purification of therapeutic proteins is derived. This is based on the equilibrium theory, which assumes instantaneous equilibrium conditions. As the MCSGP process allows protein separation with a linear modifier gradient, all equations are derived in terms of the covered distance as a function of the modifier concentration in ion-exchange chromatography. All constraints, which need to be fulfilled in order to obtain a stable process with maximum yield and purity, are described as a function of the different process parameters. For operation parameters within the parameter space where all constraints are fulfilled, a stable process is predicted. Additionally, on the boundary of this region, the optimal operation point in terms of buffer consumption and productivity can be found. Besides, the presented design space can help to analyze the impact of different process parameters on performance and stability and therefore to establish conditions for a robust operation of the process.

Publisher URL: http://dx.doi.org/10.1021/acs.iecr.7b00569

DOI: 10.1021/acs.iecr.7b00569

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.