3 years ago

Programming Cell Adhesion for On-Chip Sequential Boolean Logic Functions

Programming Cell Adhesion for On-Chip Sequential Boolean Logic Functions
Lihua Wang, Shaopeng Wang, Zhilei Ge, Shiping Song, Guangbao Yao, Chunhai Fan, Jianbang Wang, Hao Pei, Jiang Li, Xiangmeng Qu, Jiye Shi, Li Li, Xiaolei Zuo
Programmable remodelling of cell surfaces enables high-precision regulation of cell behavior. In this work, we developed in vitro constructed DNA-based chemical reaction networks (CRNs) to program on-chip cell adhesion. We found that the RGD-functionalized DNA CRNs are entirely noninvasive when interfaced with the fluidic mosaic membrane of living cells. DNA toehold with different lengths could tunably alter the release kinetics of cells, which shows rapid release in minutes with the use of a 6-base toehold. We further demonstrated the realization of Boolean logic functions by using DNA strand displacement reactions, which include multi-input and sequential cell logic gates (AND, OR, XOR, and AND-OR). This study provides a highly generic tool for self-organization of biological systems.

Publisher URL: http://dx.doi.org/10.1021/jacs.7b04040

DOI: 10.1021/jacs.7b04040

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