Journal of the American Chemical Society
Journal of the American Chemical Society
5 years ago

Genetically Encoded Photoaffinity Histone Marks

Genetically Encoded Photoaffinity Histone Marks
Peng R. Chen, Xiao Xie, Xiucong Bao, Xiang David Li, Haiping Song, Jianwei Lin, Rongfeng Zhu, Fangfei Qin, Jingyi Zhao, Xiao-Meng Li, Gong Zhang
Posttranslational modifications (PTMs) of lysine are crucial histone marks that regulate diverse biological processes. The functional roles and regulation mechanism of many newly identified lysine PTMs, however, remain yet to be understood. Here we report a photoaffinity crotonyl lysine (Kcr) analogue that can be genetically and site-specifically incorporated into histone proteins. This, in conjunction with the genetically encoded photo-lysine as a “control probe”, enables the capture and identification of enzymatic machinery and/or effector proteins for histone lysine crotonylation.

Publisher URL: http://dx.doi.org/10.1021/jacs.7b01431

DOI: 10.1021/jacs.7b01431

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