4 years ago

Brief Report: 3D Culture Reduces Cell Size By Increasing Vesicle Excretion

Brief Report: 3D Culture Reduces Cell Size By Increasing Vesicle Excretion
Ying Zhou, Sen Li, Yaojiong Wu, Miaohua Mo
Our previous study has shown that three-dimensional (3D) culture decreases mesenchymal stem cell (MSC) size, leading to enhanced trafficking ability and reduced lung vascular obstructions. However, the underlying mechanisms are unclear. In this study, we proposed that 3D culture reduces MSC size by increasing vesicle excretion. Scanning electron microscope showed that 3D culture markedly increased the amount of membrane-bound vesicles on the cell surface. In consistence, tunable resistive pulse sensing (TRPS) quantifying analysis of vesicles in the culture medium indicated that there were higher levels of vesicles in the 3D culture MSC medium. 3D culture significantly lowered the level of actin polymerization (F-actin), suggestive of lowering actin skeleton tension may facilitate vesicle excretion. Indeed, treatment of MSCs with Cytochalasin D or functional blockade of integrin β1 caused increased vesicle secretion and decreased cell sizes. Thus our results suggest that 3D culture reduces MSC size by increasing vesicle excretion which is likely mediated by lowering cytoskeleton tension. This article is protected by copyright. All rights reserved. Three-dimensional (3D) culture of mesenchymal stem cells decreases their expression of integrins on the surface, causing actin depolymerization and reduced levels of F-actin. This leads to increased excretion of extracellular vesicles (EVs), resulting in reduced MSC size.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/stem.2729

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