3 years ago

Dual control of ROS1-mediated active DNA demethylation by the DNA DAMAGE BINDING protein 2 (DDB2)

Teresa Roldán Arjona, Jean Molinier, Rafael R. Ariza, Dolores Córdoba-Cañero, Valérie Cognat
DNA methylation contributes to key regulatory processes during plant development by controlling gene expression. Genomic methylation patterns are dynamic and must be properly maintained and/or re-established upon DNA replication and active removal, therefore requiring sophisticated control mechanisms. Here, we identified a direct interplay between the DNA repair factor DNA DAMAGE BINDING protein 2 (DDB2) and the ROS1-mediated active DNA demethylation pathway in Arabidopsis thaliana. We showed that DDB2 forms a complex with ROS1 and AGO4 and that they act at ROS1 locus to modulate DNA methylation levels and therefore ROS1 expression. We also found that DDB2 represses ROS1 enzymatic activity. DNA demethylation intermediates generated by ROS1 are processed by the DNA 3′-phosphatase ZDP and the AP endonuclease APE1L, and we also showed that DDB2 interacts which both enzymes and stimulates their activities. Taken together, our results indicate that DDB2 acts as a critical regulator of ROS1-mediated active DNA demethylation. This article is protected by copyright. All rights reserved.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/tpj.13753

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