3 years ago

ENO1 (alpha-enolase) Regulates HBV Replication through Suppression of the IFN Signaling Pathway

Hu Yan-Chao, Ma Li-Na, Yan Ting-Ting, Yang Xiao-qing, Yang Yi-Xuan, Liu Xiao-Yan, Lu Zhen-Hui, Luo Xia, Ding Xiang-Chun
Persistent chronic infection with hepatitis B virus (HBV) is a major risk factor for the development of HBV-related diseases. The molecular mechanisms that underlie HBV infection and associated carcinogenesis are not fully understood. The aim of this study was to explore the role of ENO1 in HBV replication processes.Here,we examined ENO1 expression levels in HBV-infected and non-HBV-infected liver tissues and cells by Western blot analysis, real-time PCR, and immunohistochemistry. In addition, HBsAg and HBeAg in the media of transfected HepG2.2.15 cells were detected using an electrochemical luminescence analyzer within 48 hours after ENO1-specific siRNA transfection. The expression levels of HBV DNA, type I interferon and five downstream IFN-stimulated genes in HepG2.2.15 cells were examined using real-time PCR.We found ENO1 expression was upregulated in the HBV-infected liver tissues and cells. Silencing of ENO1 resulted in a significant reduction in HBV replication and this siRNA-mediated reaction also caused the upregulation of expression of type I interferon and downstream IFN-stimulated genes.Therefore,we come to the conclusion ENO1 is involved in HBV replication. It is therefore likely that HBV replication is enhanced following suppression of the IFN signaling pathway. However, the mechanisms that underpin ENO1-mediated modulation of the IFN signaling pathway remain to be elucidated. This article is protected by copyright. All rights reserved.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/jvh.12813

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.