3 years ago

HID-1 controls large dense core vesicle formation by influencing cargo sorting and TGN acidification.

Blake H Hummer, Christian Burns, Matthew S Joens, Lan Chen, Noah F de Leeuw, Cedric S Asensio, James A J Fitzpatrick, Bethany Hosford
Large dense core vesicles (LDCVs) mediate the regulated release of neuropeptides and peptide hormones. They form at the trans-Golgi network (TGN) where their soluble content aggregates to form a dense core, but the mechanisms controlling biogenesis are still not completely understood. Recent studies have implicated the peripheral membrane protein HID-1 in neuropeptide sorting and insulin secretion. Using CRISPR/Cas9, we generated HID-1 KO rat neuroendocrine cells, and show that the absence of HID-1 results in specific defects in peptide hormone and monoamine storage and regulated secretion. Loss of HID-1 causes a reduction in the number of LDCVs and affects their morphology and biochemical properties due to impaired cargo sorting and dense core formation. HID-1 KO cells also exhibit defects in TGN acidification together with mislocalization of the Golgi-enriched vacuolar H(+)-ATPase subunit isoform a2. We propose that HID-1 influences early steps in LDCV formation by controlling dense core formation at the TGN.

Publisher URL: http://doi.org/10.1091/mbc.E17-08-0491

DOI: 10.1091/mbc.E17-08-0491

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