3 years ago

Characterization of Hypericum perforatum polysaccharides with antioxidant and antimicrobial activities: Optimization based statistical modeling

Characterization of Hypericum perforatum polysaccharides with antioxidant and antimicrobial activities: Optimization based statistical modeling
In this study, the extracting parameters of crude polysaccharides (CPSs) from the Hypericum perforatum (HP) including extraction time (ETi, 60–180min), extraction temperature (ETe, 60–90°C), and the water/solid ratio (W/S, 10–30 was optimized by using three-variable-three-level Box-Behnken design-response surface methodology (BBD-RSM) based on the single-factor experiments. The optimal extraction conditions were as follow: ETi 117.5min, ETe 74.28°C, and W/S 20.3:1. Under these conditions, the experimental yield was 6.69%. Fourier transform-infrared spectroscopy (FT-IR) was used to identify structure of polysaccharide extracted at the optimal operating point. HP-CPSs was proved to possess antioxidant activities including 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and OH free-radicals scavenging activates in vitro. The antibacterial activities of HP-CPSs against Escherichia coli, Shigella dysenteriae and Salmonella typhi, Bacillus cereus and Staphylococcus aureus were evaluated by determining clear growth inhibition zone diameters and by essays in liquid media. Overall, the results indicated that those polysaccharides could offer promising sources of polysaccharides for future application as antioxidant ingredients in the food industry.

Publisher URL: www.sciencedirect.com/science

DOI: S0141813017304622

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.