3 years ago

Bacillus amyloliquefaciens FZB42 repressed plant miR846 to induce systemic resistance via jasmonic acid-dependent signaling pathway

Qianqian Xu, Wenbo Chai, Ting Ding, Haiyang Jiang, Shanshan Xie, Beijiu Cheng
Bacillus amyloliquefaciens FZB42 is a kind of plant-growth-promoting rhizobacteria (PGPR) that activates induced systemic resistance (ISR) in Arabidopsis. Blocking in cyclic lipopeptides and 2,3-butanediol synthesis of FZB42, which were proved to be involved in priming ISR, resulted in abolishment of plant defense responses. To further clarify the ISR activated by PGPR at miRNA level, sRNA libraries from Arabidopsis leaves after root-irrigated with FZB42, FZB42ΔsfpΔalsS and control were constructed and sequenced. After fold-change selection, promoter analysis and target prediction, miR846-5p and miR846-3p from the same precursor were selected as candidate ISR-associated miRNA. miR846, belongs to non-conserved miRNAs, specifically exists in Arabidopsis and its function in plant defense response remains unclear. The disease severities of transgenic Arabidopsis overexpression miR846 (OEmiR846) and knockdown miR846 (STTM846) against Pseudomonas syringae DC3000 suggested that the miR846 expression level in Arabidopsis was negatively correlated with disease resistance. Moreover, miR846 in Arabidopsis Col-0 was repressed after methyl jasmonate treatment. In addition, jasmonic acid (JA) signaling related genes were up-regulated in STTM846 and stomatal apertures of STTM846 were also less than Arabidopsis Col-0 after methyl jasmonate treatment. Furthermore, the disease resistance of STTM846 transgenic Arabidopsis against pstDC3000 was blocked by adding JA biosynthetic inhibitor diethyldiethiocarbamic acid (DIECA). Taken together, our results suggested that Bacillus amyloliquefaciens FZB42 inoculation suppressed miR846 expression to induce Arabidopsis systemic resistance via JA-dependent signaling pathway. This article is protected by copyright. All rights reserved.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/mpp.12634

You might also like
Never Miss Important Research

Researcher is an app designed by academics, for academics. Create a personalised feed in two minutes.
Choose from over 15,000 academics journals covering ten research areas then let Researcher deliver you papers tailored to your interests each day.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.