3 years ago

An Arabidopsis glycine-rich plasma membrane protein enhances disease resistance in soybean.

Madan K Bhattacharyya, Yang Yang, Rishi Sumit, Micheline N Ngaki, Binod B Sahu, Bing Wang, Subodh K Srivastava
Nonhost resistance is defined as the immunity of a plant species to all nonadapted pathogen species. Arabidopsis ecotype Columbia-0 (Col-0) is nonhost to oomycete plant pathogen Phytophthora sojae and fungal plant pathogen Fusarium virguliforme that are pathogenic to soybean. Previously, we reported generating pss1 mutation in the pen1-1 genetic background, genetic mapping and characterization of the Arabidopsis nonhost resistance Phytophthora sojae susceptible gene locus, PSS1. In this study, we identified six candidate PSS1 genes by comparing single nucleotide polymorphisms (SNPs) of (i) the bulked DNA sample of seven F2:3 families homozygous for the pss1 allele and (ii) the pen1-1 mutant with Col-0. Analyses of T-DNA insertion mutants for each of these candidate PSS1 genes identified the At3g59640 gene encoding a glycine rich protein as the putative PSS1 gene. Later, complementation analysis confirmed the identity of At3g59640 as the PSS1 gene. PSS1 is induced following P. sojae infection as well as expressed in an organ-specific manner. Co-expression analysis of the available transcriptomic data followed by reverse transcriptase PCR suggested that PSS1 is co-regulated with ATG8a (At4g21980), a core gene in autophagy. PSS1 contains a predicted single membrane spanning domain. Subcellular localization study indicated that it is an integral plasma membrane protein. Sequence analysis suggested that soybean unlikely contains a PSS1-like defense function. Following introduction of PSS1 into the soybean cultivar 'Williams 82', the transgenic plants exhibited enhanced resistance to F. virguliforme, the pathogen that causes sudden death syndrome.

Publisher URL: http://doi.org/10.1104/pp.16.01982

DOI: 10.1104/pp.16.01982

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