3 years ago

Complete genome sequence of a novel, virulent Ahjdlikevirus bacteriophage that infects Enterococcus faecium

Zhiqiang Mi, Qiang Sun, Yigang Tong, Shaozhen Xing, Kaifei Fu, Xiaoping An, Guangqian Pei, Jian Wang, Lijun Zhou, Xianglilan Zhang, Yong Huang, Baohua Zhao

Abstract

A novel virulent bacteriophage named vB_EfaP_IME199 that specifically infects Enterococcus faecium was isolated and characterized. Its optimal multiplicity of infection was 0.01, and it had a 30 minute outbreak period. High-throughput sequencing revealed that the phage has a dsDNA genome of 18,838 bp with 22 open reading frames. The genome has very low homology to all other bacteriophage sequences in the GenBank database. Run-off sequencing experiments confirmed that vB_EfaP_IME199 has short inverted terminal repeats. Phylogenetic analysis indicated that vB_EfaP_IME199 can be taxonomically classified as a new member of the genus Ahjdlikevirus of family Podoviridae.

Publisher URL: https://link.springer.com/article/10.1007/s00705-017-3503-1

DOI: 10.1007/s00705-017-3503-1

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