3 years ago

Identification of a novel envelope protein encoded by ORF 136 from Cyprinid herpesvirus 3

Weiwei Zeng, Shucheng Zheng, Qing Wang, Yingying Li, Sven M. Bergmann, Cunbin Shi, Yingying Wang, Yan Ren, Jiexing Wu

Abstract

Cyprinid herpesvirus 3 (CyHV-3) is the pathogenic agent of koi herpesvirus disease (KHVD) afflicting common carp and koi (Cyprinus carpio L.) populations globally. As described previously, proteomic analyses of purified CyHV-3 particles have shown that at least 46 structural proteins are incorporated into CyHV-3 virions; among these ORF136 may encode a putative envelope protein. In this study, Western blotting analysis showed that a specific band with the predicted molecular weight of 17 kDa was detected both in purified virions and envelope components using a rabbit anti-ORF136 polyclonal antibody. Indirect immunofluorescence assay with confocal laser scanning microscopy indicated that the ORF136 protein was distributed in the cytoplasm of CCB cells infected with CyHV-3 and transfected with a pVAX1-ORF136 plasmid. Furthermore, immunogold electron microscopy confirmed that ORF136 protein localized to the CyHV-3 envelope.

Publisher URL: https://link.springer.com/article/10.1007/s00705-017-3528-5

DOI: 10.1007/s00705-017-3528-5

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