3 years ago

The effect of temporal manipulation of TGFβ3 and FGF2 on the derivation of proliferative chondrocytes from Mensenchymal Stem Cells – a study monitored by qRT-PCR and Molecular Beacon based Nanosensors

Christian Wiraja, Chenjie Xu, Yingnan Wu, Li Min Tay, Eng Hin Lee, Zheng Yang
Proliferative chondrocytes are critical to realize regeneration of damaged epiphyseal growth plate. However, acquiring autologous replacement cells involves highly-invasive procedures and often results in limited cell quantity. Mesenchymal stem cells (MSCs) are a potential source of chondrogenic cells for the treatment of cartilage disorders and injuries. The temporal effect of transforming growth factor beta 3 (TGFβ3) and fibroblast growth factor 2 (FGF2) on the derivation of proliferative chondrocytes from MSCs in 3D agarose was investigated by manipulating the duration of TGFβ3 and FGF2 treatment. The differentiation process was monitored by quantitative reverse transcription polymerase chain reaction (qRT-PCR) as well as nanosensors containing two molecular beacons that target critical biomarkers for proliferative chondrocytes (i.e. collagen type-II mRNA and Ki67 mRNA). The molecular beacon-based nanosensors were found to be comparable to qRT-PCR in measuring mRNA expression and thus providing a non-invasive mean to screen and monitor culture samples. This article is protected by copyright. All rights reserved.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/jbm.a.36286

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