3 years ago

A color-code for glycosaminoglycans identification by means of polyacrylamide gel electrophoresis stained with the cationic carbocyanine dye Stains-all

Ana Maria Freire Tovar, João Pedro Souza Andrade, Eduardo Vilanova, Paulo Antonio de Souza Mourão, Caroline Pacheco Oliveira
Cationic dyes such as toluidin blue are commonly employed to visualize Glycosaminoglycans (GAGs) on electrophoresis gels; however, the carbocyanine based dye Stains-all has been increasingly used to stain the non-sulfated hyaluronic acid and other GAGs in submicrogram quantities. In this short communication, we demonstrate that Stains-all is able to stain the most common GAGs on polyacrylamide gels with distinct and contrasting colors in a reproducible manner. We also show that this staining method is useful to identify GAGs present both in mixtures and in submicrogram quantities. Therefore, Stains-all has shown to be useful in identifying GAGs on polyacrylamide gels with basis on their specific colors, at least on a screening level. This article is protected by copyright. All rights reserved

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/elps.201700391

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