3 years ago

Thermophilic Tetramolecular G-quadruplex/Hemin DNAzyme

David Monchaud, Huangxian Ju, Jielin Chen, Yuehua Guo, Jun Zhou, Mingpan Cheng
Quadruplex-based DNAzyme system is one of the most useful artificial enzymes or catalysts due to their unique properties that make them reliable alternatives to proteins for performing catalytic transformation. Herein, we report on the first prototype of a thermally stable DNAzyme system, referred to as a thermophilic DNAzyme, capable of oxidizing substrates in harsh experimental conditions, i.e., high temperature (up to 95 °C) and long reaction times (up to 18 h at 75 °C). The step-by-step design of this unique heat-activated G-quadruplex/hemin catalyst, including the modification of adenines at both ends of G-tracts, the choice of cation and its concentration for DNAzyme stabilization, is described. This work investigates thoroughly the molecular basis of these catalytic properties and provides an example of an industrially relevant application.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201708964

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