3 years ago

Parquetina nigrescens leaves: chemical profile and influence on the physical and biochemical indices of sexual activity of male Wistar rats

The leaves of Parquetina nigrescens have been claimed in folk medicine to be useful for managing sexual dysfunction, but there is inadequate scientific evidence for this claim. This investigation was conducted to assess the effects of aqueous leaf extract of Parquetina nigrescens (AEPN) in rats induced with sexual dysfunction. Methods Male rats were allocated into various groups after being induced into sexual dysfunction with paroxetine hydrochloride. The groups were treated with distilled water, PowMaxM (reference drug), and the AEPN at 20, 40 and 80 mg/kg body weight (BW) respectively for 7 d. Frequencies of mount (MF), intromission (IF), ejaculation (EF), as well as latencies of mount (ML), intromission (IL) and post – ejaculatory interval (PEI) were progressively monitored with receptive female rats on days 1, 3 and 7. The precentage of mounted, intromitted, and ejaculated rats, intromission ratio (IR), copulatory efficiency (CE) and intercopulatory interval (ICI) were also computed. Standard methods were employed to determine the levels of serum luteinizing and follicle stimulating hormones (LH and FSH), testosterone, nitric oxide (NO) and the activity of phosphodiesterase V (PDE5). Results The plant contained alkaloids, saponins, flavonoids, cardiac glycosides, steroids, tannins, phlobatannins, cardenolides, phenolics, anthraquinones and triterpenes with alkaloids (2.32 mg/g) occurring in greatest quantity while flavonoids, anthraquinones and cardenolides (0.01 mg/g, each) were the least. All the 20 standard amino acids were detected in the plant leaf, with the lowest concentration being 0.30 mg/g for methionine and the highest being 2.12 mg/g for cysteine. Furthermore, P. nigrescens leaves contained Na, K, Fe, Mg, Zn, Ca, Cu, Mn, P, Pb, Cd, Ni, Cr, and Co, with Fe present in the highest and Co the lowest concentrations. Paroxetine significantly (P<0.05) reduced MF, IF, EF, percentage mounted, intromitted, ejaculated, IR, CE, serum testosterone, LH, FSH and NO, while ML, IL, PEI, IF and PDE5 were significantly (P<0.05) increased and/or prolonged. The AEPN significantly reversed these paroxetine-treatment-related alterations with most profound activity at the highest dose of 80 mg/kg BW, similar to that of the PowMaxM-treated animals. Conclusion The AEPN restored both the physical and biochemical indices of male sexual activity/competence via changes in reproductive hormones, NO and PDE5 activity. The pro-sexual activity, attributed to a myriad of mineral, amino acid and secondary metabolite constituents, was best at 80 mg/kg BW of AEPN.

Publisher URL: www.sciencedirect.com/science

DOI: S2095496417603182

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.