3 years ago

Self-Replicating Catalyzed Hairpin Assembly for Rapid Signal Amplification

Self-Replicating Catalyzed Hairpin Assembly for Rapid Signal Amplification
Dan Xiao, Jianyuan Dai, Yong Guo, Hongfei He, Zhijuan Duan
A rapid signal amplification system based on the self-replicating catalyzed hairpin assembly is reported in which two hairpins, H1 and H2, were well-designed in which two split target/trigger DNA and two split G-quadruplex sequences were respectively integrated into H1 and H2. Target/trigger DNA can be cyclically used in this system to form the duplex DNA assemblies (H1–H2), which will bring the two G-quadruplex fragments into close-enough proximity to induce the formation of intact G-quadruplex as a colorimetric signal readout. Similarly, the two split target/trigger DNA sequences will reunite into a DNA sequence that is identical to the target/trigger DNA; then, the obtained replica can also be cyclically used as a new activator unit to trigger the CHA reaction, leading to the rapidly and significantly enhanced formation of target/trigger DNA replicas with the concomitant generation of a higher signal. This self-replication-based autocatalytic signal amplified approach has been successfully used to develop a rapid and visual assay for DNA and small molecule detection.

Publisher URL: http://dx.doi.org/10.1021/acs.analchem.7b01946

DOI: 10.1021/acs.analchem.7b01946

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