5 years ago

Enhanced Fibrinolysis with Magnetically Powered Colloidal Microwheels

Enhanced Fibrinolysis with Magnetically Powered Colloidal Microwheels
Keith B. Neeves, David W. M. Marr, Paco S. Herson, Dante Disharoon, Tonguc O. Tasci, Kuldeepsinh Rana, Rogier M. Schoeman
Thrombi that occlude blood vessels can be resolved with fibrinolytic agents that degrade fibrin, the polymer that forms between and around platelets to provide mechanical stability. Fibrinolysis rates however are often constrained by transport-limited delivery to and penetration of fibrinolytics into the thrombus. Here, these limitations are overcome with colloidal microwheel (µwheel) assemblies functionalized with the fibrinolytic tissue-type plasminogen activator (tPA) that assemble, rotate, translate, and eventually disassemble via applied magnetic fields. These microwheels lead to rapid fibrinolysis by delivering a high local concentration of tPA to induce surface lysis and, by taking advantage of corkscrew motion, mechanically penetrating into fibrin gels and platelet-rich thrombi to initiate bulk degradation. Fibrinolysis of plasma-derived fibrin gels by tPA-microwheels is fivefold faster than with 1 µg mL−1 tPA. µWheels following corkscrew trajectories can also penetrate through 100 µm sized platelet-rich thrombi formed in a microfluidic model of hemostasis in ≈5 min. This unique combination of surface and bulk dissolution mechanisms with mechanical action yields a targeted fibrinolysis strategy that could be significantly faster than approaches relying on diffusion alone, making it well-suited for occlusions in small or penetrating vessels not accessible to catheter-based removal. Microwheels use a combined mechanical and biochemical mechanism to lyse blood clots. Superparamagnetic particles functionalized with the fibrinolytic agent tissue plasminogen activator are introduced into an occluded channel and, upon application of a magnetic field, self-assemble into wheel-like microbots capable of translating to and penetrating through blood clots.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/smll.201700954

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