3 years ago

Engineering of a DNA polymerase for direct m6A sequencing

Martina Adam, Virginie Marchand, Andreas Marx, Mark Helm, Yuri Motorin, Stephan Werner, Joos Aschenbrenner
Methodologies for the detection of RNA modifications are of fundamental importance for advancing epitranscriptomics. N6-methyladenosine (m6A) is the most abundant RNA modification in mammalian mRNA and involved in regulation of gene expression. Current detection techniques are laborious and rely on antibody based enrichment of m6A containing RNA prior to sequencing, as m6A modifications are generally "erased" during reverse transcription (RT). To overcome the drawbacks associated with indirect detection, we aimed to generate novel DNA polymerase variants for direct m6A sequencing. Therefore, we developed a screen to evolve an RT-active KlenTaq DNA polymerase variant that sets a mark for N6-methylation. We identified a mutant that exhibits increased misincorporation opposite m6A as compared to unmodified A. Application of the generated DNA polymerase in Next-Generation Sequencing allowed the identification of m6A sites directly from the sequencing data of untreated RNA samples.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201710209

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