3 years ago

Luciferases with Tunable Emission Wavelengths

Luciferases with Tunable Emission Wavelengths
Rudolf Griss, Marina D.B. Sabbadini, Kai Johnsson, Luc Reymond, Qiuliyang Yu, Lin Xue, Julien Hiblot, Nicholas Hill, Olivier Sallin, Alberto Schena
We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP-tag or HaloTag7. Labeling of the self-labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Luciferases with tunable colors have applications as reporter genes, for the construction of biosensors and in bioimaging. Shining chimeras: Merging the luciferase NanoLuc with the self-labeling proteins tags SNAP-tag or HaloTag7 yields luciferases whose colors can be freely tuned by incubating them with fluorescent probes in vitro or in cellulo. Luciferases with tunable luminescence have applications as reporter genes and open up novel opportunities in bioimaging and biosensing.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201708277

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