3 years ago

Effector-Triggered Self-Replication in Coupled Subsystems

Effector-Triggered Self-Replication in Coupled Subsystems
Gaël Schaeffer, Sijbren Otto, Meniz Tezcan, Dávid Komáromy, Ivana Marić
In living systems processes like genome duplication and cell division are carefully synchronized through subsystem coupling. If we are to create life de novo, similar control over essential processes such as self-replication need to be developed. Here we report that coupling two dynamic combinatorial subsystems, featuring two separate building blocks, enables effector-mediated control over self-replication. The subsystem based on the first building block shows only self-replication, whereas that based on the second one is solely responsive toward a specific external effector molecule. Mixing the subsystems arrests replication until the effector molecule is added, resulting in the formation of a host–effector complex and the liberation of the building block that subsequently engages in self-replication. The onset, rate and extent of self-replication is controlled by the amount of effector present. An effector molecule triggers self-replication in a mixed dynamic combinatorial library constructed from two coupled subsystems, the first devoted to effector recognition and the second to self-replication. The approach is modular and allows for quantitative control over the self-replication process.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201707191

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