5 years ago

Alternative Affinity Ligands for Immunoglobulins

Alternative Affinity Ligands for Immunoglobulins
Tomaž Bratkovič, Nika Kruljec
The demand for recombinant therapeutic antibodies and Fc-fusion proteins is expected to increase in the years to come. Hence, extensive efforts are concentrated on improving the downstream processing. In particular, the development of better-affinity chromatography matrices, supporting robust time- and cost-effective antibody purification, is warranted. With the advances in molecular design and high-throughput screening approaches from chemical and biological combinatorial libraries, novel affinity ligands representing alternatives to bacterial immunoglobulin (Ig)-binding proteins have entered the scene. Here, we review the design, development, and properties of diverse classes of alternative antibody-binding ligands, ranging from engineered versions of Ig-binding proteins, to artificial binding proteins, peptides, aptamers, and synthetic small-molecular-weight compounds. We also provide examples of applications for the novel affinity matrices in chromatography and beyond.

Publisher URL: http://dx.doi.org/10.1021/acs.bioconjchem.7b00335

DOI: 10.1021/acs.bioconjchem.7b00335

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