Biotechnology Journal
Biotechnology Journal
4 years ago

Cover Picture: Biotechnology Journal 11/2017

Cover Picture: Biotechnology Journal 11/2017
A new method has been developed for efficient production of soluble proteins in a cell-free synthesis system. This method involves the use of an engineered ubiquitin sequence as an N-terminal sequence element that enhances both the productivity and solubility of recombinant proteins. Recombinant proteins were produced with their native amino acid sequences through in situ removal of ubiquitin during cell-free synthesis reactions in the presence of a deubiquitinase. The presented strategy could be employed as a facile route to prepare soluble proteins required for various applications. This is reported by Devi Kasi, Hee Ju Nah, Christy Catherine, Eung-Soo Kim, Kyubeom Han, Jong-Cheon Ha and Dong-Myung Kim, article number 1700125.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/biot.201770111

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.