5 years ago

Diluted seawater affects phytohormone receptors and maintain the protonema stage in Physcomitrella patens

Li Huan, Zhenbing Zheng, Guang-Ce Wang, Shan Gao
Due to its highly efficient homologous recombination ability and unusual evolutionary position, the moss Physcomitrella patens has begun to attract more attention in genetic and evolutionary studies. Protonema, the filament stage of the gametophyte is of great significance in P. patens protoplast isolation. Moreover, protonema is widely used in genetic engineering. However, difficulties in the induction and state maintenance of protonema restrict its wider application. In this work, protonema was induced efficiently in a diluted seawater medium, and the filamentous state was maintained without further cell differentiation. The developmental process of the protonema resumed, progressing to bud assembly and gametophore formation after transfer to fresh water medium. In addition, a transcriptome analysis showed that plant hormone signal transduction pathways were down-regulated when protonema was grown in diluted seawater medium. Consistent with the transcriptome results, the protonema failed to respond to addition of indole-3-acetic acid (IAA) and 6-benzylaminopurine (6-BA) to the diluted seawater medium. Based on these results, we concluded that diluted seawater medium block the differentiation of protonema. This result could provide a novel insight to benefit future protonema production. This article is protected by copyright. All rights reserved.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/tpj.13764

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